scott gradia plic his mbp tev tbrucei h1 1 (Addgene inc)
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scott gradia plic his mbp tev tbrucei h1 1
Scott Gradia Plic His Mbp Tev Tbrucei H1 1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 99 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pet+his6+mbp+tev/pET+His6+MBP+TEV+LIC+cloning+vector+(1M)+(Plasmid+%2329656)/pm41709455-917-215-213
Average 94 stars, based on 99 article reviews
Scott Gradia Plic His Mbp Tev Tbrucei H1 1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 99 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pet+his6+mbp+tev/pET+His6+MBP+TEV+LIC+cloning+vector+(1M)+(Plasmid+%2329656)/pm41709455-917-215-213
Average 94 stars, based on 99 article reviews
scott gradia plic his mbp tev tbrucei h1 1 - by Bioz Stars,
2026-10
94/100 stars
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Polymerase Chain Reaction:Article Title: Drosophila FMRP recruits the miRISC to target mRNAs to repress translation Article Snippet: To construct the FLAG-tagged GW182 plasmid, the CDS for GW182 was PCR amplified from LD47780 (DGRC) and inserted into the pAFW vector (DGRC) by Gateway Cloning (Invitrogen). .. For the EMSA experiments, the dFMRP CDS was PCR amplified from pAc5.1-EGFP-dFMRP and transferred into Article Title: FMRP cooperates with miRISC components to repress translation and regulate neurite morphogenesis in Drosophila Article Snippet: To construct the FLAG-tagged GW182 plasmid, the CDS for GW182 was PCR amplified from LD47780 (DGRC) and inserted into the pAFW vector (DGRC) by Gateway Cloning (Invitrogen). .. For the EMSA experiments, the dFMRP CDS was PCR amplified from pAc5.1-EGFP-dFMRP and transferred into Amplification:Article Title: Drosophila FMRP recruits the miRISC to target mRNAs to repress translation Article Snippet: To construct the FLAG-tagged GW182 plasmid, the CDS for GW182 was PCR amplified from LD47780 (DGRC) and inserted into the pAFW vector (DGRC) by Gateway Cloning (Invitrogen). .. For the EMSA experiments, the dFMRP CDS was PCR amplified from pAc5.1-EGFP-dFMRP and transferred into Article Title: FMRP cooperates with miRISC components to repress translation and regulate neurite morphogenesis in Drosophila Article Snippet: To construct the FLAG-tagged GW182 plasmid, the CDS for GW182 was PCR amplified from LD47780 (DGRC) and inserted into the pAFW vector (DGRC) by Gateway Cloning (Invitrogen). .. For the EMSA experiments, the dFMRP CDS was PCR amplified from pAc5.1-EGFP-dFMRP and transferred into Ligation:Article Title: Drosophila FMRP recruits the miRISC to target mRNAs to repress translation Article Snippet: To construct the FLAG-tagged GW182 plasmid, the CDS for GW182 was PCR amplified from LD47780 (DGRC) and inserted into the pAFW vector (DGRC) by Gateway Cloning (Invitrogen). .. For the EMSA experiments, the dFMRP CDS was PCR amplified from pAc5.1-EGFP-dFMRP and transferred into Article Title: FMRP cooperates with miRISC components to repress translation and regulate neurite morphogenesis in Drosophila Article Snippet: To construct the FLAG-tagged GW182 plasmid, the CDS for GW182 was PCR amplified from LD47780 (DGRC) and inserted into the pAFW vector (DGRC) by Gateway Cloning (Invitrogen). .. For the EMSA experiments, the dFMRP CDS was PCR amplified from pAc5.1-EGFP-dFMRP and transferred into Cloning:Article Title: Drosophila FMRP recruits the miRISC to target mRNAs to repress translation Article Snippet: To construct the FLAG-tagged GW182 plasmid, the CDS for GW182 was PCR amplified from LD47780 (DGRC) and inserted into the pAFW vector (DGRC) by Gateway Cloning (Invitrogen). .. For the EMSA experiments, the dFMRP CDS was PCR amplified from pAc5.1-EGFP-dFMRP and transferred into Article Title: FMRP cooperates with miRISC components to repress translation and regulate neurite morphogenesis in Drosophila Article Snippet: To construct the FLAG-tagged GW182 plasmid, the CDS for GW182 was PCR amplified from LD47780 (DGRC) and inserted into the pAFW vector (DGRC) by Gateway Cloning (Invitrogen). .. For the EMSA experiments, the dFMRP CDS was PCR amplified from pAc5.1-EGFP-dFMRP and transferred into Clone Assay:Article Title: PLK1-Mediated Phosphorylation Cascade Activates the Mis18 Complex to Ensure Centromere Inheritance Article Snippet: Codon optimised (GeneArt) Mis18α and Mis18β genes were cloned into expression vectors pET His6 TEV (9B) and pET His6 msfGFP TEV (9GFP, Addgene plasmids #48284 and #48287, a gift from Scott Gradia), respectively, and combined to form a polycistronic vector. .. Mis18BP1 1-490 was cloned from a codon optimised sequence (GeneArt) into the Article Title: Structural Basis for Mis18 Complex Assembly: Implications for Centromere Maintenance Article Snippet: .. Mis18BP1 20-130 was cloned in pEC-K-3C-His-GST and Article Title: Structural basis for Mis18 complex assembly and its implications for centromere maintenance Article Snippet: .. The boundaries of ΔN for Mis18α and Mis18β were 77–187 and 56–183 Mis18BP1 20-130 was cloned in pEC-K-3C-His-GST and Article Title: Molecular basis for Cdk1‐regulated timing of Mis18 complex assembly and CENP‐A deposition Article Snippet: Human Mis18α, Mis18α MeDiY , Mis18α C‐term , Mis18β, Mis18β MeDiY , and Mis18β C‐term were amplified from codon‐optimized sequences (GeneArt) while Mis18BP1 20–130 was amplified from a human cDNA library (MegaMan human transcriptome library, Agilent). .. Amplifications were then cloned into pET His6 msfGFP TEV, pET His6 TEV, pET His6 Sumo TEV, pET His6 MBP TEV, pGEX‐6P‐1 (GE Healthcare), pEC‐K‐3C‐His‐GST, and pEC‐K‐3C‐His LIC vectors. pET His6 msfGFP TEV (9GFP Addgene plasmid # 48287), pET His6 TEV (9B Addgene plasmid # 48284), pET His6 Sumo TEV (14S Addgene plasmid # 48291), and Sequencing:Article Title: PLK1-Mediated Phosphorylation Cascade Activates the Mis18 Complex to Ensure Centromere Inheritance Article Snippet: Codon optimised (GeneArt) Mis18α and Mis18β genes were cloned into expression vectors pET His6 TEV (9B) and pET His6 msfGFP TEV (9GFP, Addgene plasmids #48284 and #48287, a gift from Scott Gradia), respectively, and combined to form a polycistronic vector. .. Mis18BP1 1-490 was cloned from a codon optimised sequence (GeneArt) into the Plasmid Preparation:Article Title: PLK1-Mediated Phosphorylation Cascade Activates the Mis18 Complex to Ensure Centromere Inheritance Article Snippet: Codon optimised (GeneArt) Mis18α and Mis18β genes were cloned into expression vectors pET His6 TEV (9B) and pET His6 msfGFP TEV (9GFP, Addgene plasmids #48284 and #48287, a gift from Scott Gradia), respectively, and combined to form a polycistronic vector. .. Mis18BP1 1-490 was cloned from a codon optimised sequence (GeneArt) into the Article Title: Structural Basis for Mis18 Complex Assembly: Implications for Centromere Maintenance Article Snippet: .. Mis18BP1 20-130 was cloned in pEC-K-3C-His-GST and Article Title: Structural basis for Mis18 complex assembly and its implications for centromere maintenance Article Snippet: .. The boundaries of ΔN for Mis18α and Mis18β were 77–187 and 56–183 Mis18BP1 20-130 was cloned in pEC-K-3C-His-GST and Article Title: Molecular basis for Cdk1‐regulated timing of Mis18 complex assembly and CENP‐A deposition Article Snippet: Human Mis18α, Mis18α MeDiY , Mis18α C‐term , Mis18β, Mis18β MeDiY , and Mis18β C‐term were amplified from codon‐optimized sequences (GeneArt) while Mis18BP1 20–130 was amplified from a human cDNA library (MegaMan human transcriptome library, Agilent). .. Amplifications were then cloned into pET His6 msfGFP TEV, pET His6 TEV, pET His6 Sumo TEV, pET His6 MBP TEV, pGEX‐6P‐1 (GE Healthcare), pEC‐K‐3C‐His‐GST, and pEC‐K‐3C‐His LIC vectors. pET His6 msfGFP TEV (9GFP Addgene plasmid # 48287), pET His6 TEV (9B Addgene plasmid # 48284), pET His6 Sumo TEV (14S Addgene plasmid # 48291), and |